University of Michigan
Microfluidic Tools to Model and Analyze the Body
Location: EB I Room 1011
Friday, April 5th 2013 - 11:00 am
Many biological studies and pharmacological assays require culture and manipulation of living cells outside of their natural environment in the body. The gap between the cellular microenvironment in vivo and in vitro, however, poses challenges for obtaining physiologically relevant responses from cellular drug screens and for drawing out the maximum functional potential from cells used therapeutically. One of the reasons for this gap is because the fluidic environment of mammalian cells in vivo is microscale, 3D, and dynamic whereas typical in vitro cultures are macroscopic, 2D, and static. This presentation will give an overview of efforts in our laboratory to develop microfluidic systems that enable spatio-temporal control of chemical, cellular and fluid mechanical environment of cells. The technologies and methods close the physiology gap to provide biological information otherwise unobtainable and to enhance cellular performance in therapeutic applications. The seminar will also present technologies to perform biochemical analysis of protein biomarkers from small volume samples of microfluidic models of the body as well as real patients and disease. Specific biomedical topics that will be discussed include, in vitro fertilization on a chip, lung-on-a-chip, engineered 3D micro-tumors, chromatin analysis, and protein biomarker analysis.